1.5-mm spacers will produce more concentrated and denser protein bands, but 1-mm spacers may produce a more even transfer on
SOLVED: Here is a 1 gel recipe for 6% stacking gel. mL 30% acrylamide/bis-acrylamide (wear gloves and goggles! Neurotoxinl) mL 4x Tris-CI/SDS buffer pH 6.8 (1.5 M Tris-Cl pH 6.8,0.4% SDS) mL
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